Various domains of the B-cell regulatory molecule CD72 has diverged at different rates in mammals: cloning, transcription and mapping of porcine CD72
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Various domains of the B-cell regulatory molecule CD72 has diverged at different rates in mammals : cloning, transcription and mapping of porcine CD72. / Petersen, Cathrine Bie; Hillig, Ann-Britt Nygaard; Fredholm, Merete; Aasted, Bent; Salomonsen, Jan.
I: Developmental & Comparative Immunology, Bind 31, Nr. 5, 2007, s. 530-538.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Various domains of the B-cell regulatory molecule CD72 has diverged at different rates in mammals
T2 - cloning, transcription and mapping of porcine CD72
AU - Petersen, Cathrine Bie
AU - Hillig, Ann-Britt Nygaard
AU - Fredholm, Merete
AU - Aasted, Bent
AU - Salomonsen, Jan
PY - 2007
Y1 - 2007
N2 - We report the cloning of the porcine B-cell co-receptor CD72, as well as genomic mapping and examination of transcription. The B-cell receptor (BCR) complex mediates signalling upon antigen recognition by the membrane bound BCR. Several co-receptors modulate this signal positively or negatively. CD72 has been shown to be a negatively regulating BCR co-receptor. We isolated and sequenced three porcine CD72 transcript variants. Using a pig radiation hybrid panel we found the porcien CD72 gene to be located on chromosome 1q21-28 in a region syntenic to human chromosome 9. The porcine CD72 gene is highly transcribed in lymph node, thymus and lung tissues as well as in pulmonary alveolar macrophages. The predicted porcine CD72 polypeptide shows conservation of immunoreceptor tyrosine-based inhibitory motifs and an extracellular C-type lectin domain. Compared to CD72 sequences from other mammals as well as from chicken, the polypeptide is highly conserved in the intracellular part and much less conserved in the extracellular part. We suggest that this difference might be due to the different nature of ligands and the constrains on these to co-evolve.
AB - We report the cloning of the porcine B-cell co-receptor CD72, as well as genomic mapping and examination of transcription. The B-cell receptor (BCR) complex mediates signalling upon antigen recognition by the membrane bound BCR. Several co-receptors modulate this signal positively or negatively. CD72 has been shown to be a negatively regulating BCR co-receptor. We isolated and sequenced three porcine CD72 transcript variants. Using a pig radiation hybrid panel we found the porcien CD72 gene to be located on chromosome 1q21-28 in a region syntenic to human chromosome 9. The porcine CD72 gene is highly transcribed in lymph node, thymus and lung tissues as well as in pulmonary alveolar macrophages. The predicted porcine CD72 polypeptide shows conservation of immunoreceptor tyrosine-based inhibitory motifs and an extracellular C-type lectin domain. Compared to CD72 sequences from other mammals as well as from chicken, the polypeptide is highly conserved in the intracellular part and much less conserved in the extracellular part. We suggest that this difference might be due to the different nature of ligands and the constrains on these to co-evolve.
KW - Former LIFE faculty
KW - Porcine
KW - B-cell receptor
KW - Adaptive immunity
KW - CD72
U2 - 10.1016/j.dci.2006.07.008
DO - 10.1016/j.dci.2006.07.008
M3 - Journal article
C2 - 17023047
VL - 31
SP - 530
EP - 538
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
IS - 5
ER -
ID: 8043353